FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3252490
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG742312.2822821899529284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102150.035.274771
GGTATCA45550.033.127781
TATCAAC113150.031.5002382
TCAACGC115150.030.9125424
ATCAACG115400.030.7601933
GCCGTAT3300.030.43728854
CAACGCA117800.030.3288215
AACGCAG119650.029.83326
GTGGTAT19100.028.2642541
ACGAATA3550.027.55671724
AGAGTAC130050.027.44957211
ACGCAGA130600.027.3065787
CGCAGAG134150.026.559358
AGTACGG102800.026.10088213
CGTATCA4200.025.93498656
GCAGAGT137650.025.931229
CAGAGTA139100.025.80060610
TGGTATC20700.025.5846212
GAGTACG107650.025.16635112
GTACGGG112500.023.64864514
Produced by FastQC (version 0.11.5)