FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935652
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28370.3032110229016771No Hit
GTAATAAATAGCCGTGTCCTCGGCTCTCAGGTGGTTCATTTGTAGATACA26400.28215618627438405No Hit
GCGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGT25220.26954465976666536No Hit
CTACAAACGCCGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG24580.2627045097963773No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20710.22134297794479144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19300.2062732725415005No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19070.2038150936459282No Hit
CTGTTGGACACATCTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTT18900.20199817881007043No Hit
GGCCAAAAGTGCACGGCGTTTGTAGAGCTTGCATGCAGTAATAAATTCCA18230.19483739680992504No Hit
GGCACAGACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGAATTTATTACTGCATGCAAGCTCTA17500.1870353507500652No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17490.18692847340677946No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17060.18233274764549212No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16830.17987456874991983No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16800.1795539367200626No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16710.17859204063049083No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16440.17570635236177554No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG16120.1722862773766315No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAAAAGGCAC15980.17078999457063096No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15870.1696143437944877No Hit
CATCTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCC15700.16779742895862992No Hit
ATTTATTACTGTGCGAGGCCCATGAACATAAAGTCGTATTATTATGCAAT15360.16416359928691437No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15290.1634154578839141No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15140.16181229773462785No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14950.15978162821219855No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14770.15785783603305503No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT14740.15753720400319776No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTAGTGCCTTTTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG14190.15165895012248143No Hit
GGTCTAGTCAGAGCCTCCTGCATACTGTTGGACACATCTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC14140.15112456340605268No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14110.1508039313761954No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13910.1486663845104804No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13830.14781136576419437No Hit
GTCTGTGCCTGATCCACTGCCACTGAACCTGTCGGAGACCCCGGCCGCCC13790.14738385639105137No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13770.14717010170447986No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG13380.14300188531633556No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13150.14054370642076328No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12940.1382992822117625No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12790.13669612206247622No Hit
CCTGCATACTGTTGGACACATCTACTTGGATTGGTACCTGCAGAAGCCAG12690.1356273486296187No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12500.13359667910718942No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12330.13177976427133165No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12020.12846656662947334No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11800.12611526507718682No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11690.12493961430104356No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG11450.12237455806218552No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11330.12109202994275649No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA11310.120878275256185No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11300.12077139791289923No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11240.12013013385318475No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC11100.11863385104718421No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTACCCGTCACCC11010.11767195495761246No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT10980.1173513229277552No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCG10920.11671005886804069No Hit
GTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAAAATCAGCAGAG10900.11649630418146918No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10610.1133968612261824No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10610.1133968612261824No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10480.1120074557634676No Hit
GGGCTGGAGTGGGTGGCCAACATAAAAGAAGATGGAAGTGAGAGATACTA10440.1115799463903246No Hit
GACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGAATTTATTACTGCATGCAAGCTCTACAAACG10240.10944239952460957No Hit
GTCTCCACTCTCCCTACCCGTCACCCCTGGAGAGTCGGCCTCCATCTCCT10200.10901489015146656No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCGGCCGGGGTCTCC10080.10773236203203757No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10050.10741173000218031No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10050.10741173000218031No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10010.10698422062903729No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9990.1067704659424658No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9950.10634295656932277No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9880.10559481516632253No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9800.1047397964200365No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9740.10409853236032202No Hit
GTCGGAGACCCCGGCCGCCCGATTAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA9640.10302975892746448No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9520.10174723080803545No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9410.10057158003189219No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG350.00630001153.8194670
TTAGACG200.00729070951.8714639
TTCGTGT306.26975E-446.0610236
ATTTTCG306.296288E-446.0215633
GGTATCA6650.042.4839551
CGTTAAC350.001257378439.9887457
TGCGTTA350.001269167939.91291855
AGTTCGC350.001357377439.3711628
GTATCAA14000.038.6370851
GTGGTAT2150.038.4596631
CGTCAGA1500.036.7464185
TGGTATC2300.035.9495052
CCGACAA400.00245560134.8935654
ATGCGTT400.00245560134.8935654
ATGTGTA1700.034.44976811
TATCAAC15700.034.0127372
GTGTATA1800.032.5358913
TGTATAA1700.032.4233114
TCAACGC16700.031.9743354
CGTATGC652.2608008E-531.96891641
Produced by FastQC (version 0.11.5)