FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005578406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935652
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27060.28921009093124367No Hit
GCGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGT25530.2728578574085237No Hit
GTAATAAATAGCCGTGTCCTCGGCTCTCAGGTGGTTCATTTGTAGATACA23370.24977235125880135No Hit
CTACAAACGCCGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG22330.2386571075570832No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20610.22027420451193394No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18990.20296007489964216No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17540.1874628601232082No Hit
CTGTTGGACACATCTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTT17510.18714222809335096No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17390.18585969997392193No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16920.18083646483949162No Hit
GGCCAAAAGTGCACGGCGTTTGTAGAGCTTGCATGCAGTAATAAATTCCA16790.17944705937677682No Hit
ATTTATTACTGTGCGAGGCCCATGAACATAAAGTCGTATTATTATGCAAT16470.1760269843916328No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16290.17410319221248927No Hit
GGCACAGACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGAATTTATTACTGCATGCAAGCTCTA15750.16833181567505867No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15310.16362921257048563No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15100.16138478836148482No Hit
GTCTGTGCCTGATCCACTGCCACTGAACCTGTCGGAGACCCCGGCCGCCC15020.16052976961519883No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14680.15689593994348328No Hit
CATCTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCC14600.15604092119719726No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAAAAGGCAC14550.1555065344807685No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14280.15262084621205319No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14140.15112456340605268No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT14070.1503764220030524No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTAGTGCCTTTTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG13940.14898701654033764No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13780.14727697904776563No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13470.14396378140590732No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13400.14321564000290707No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13380.14300188531633556No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13340.14257437594319256No Hit
GGTCTAGTCAGAGCCTCCTGCATACTGTTGGACACATCTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC12740.13616173534604747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12590.1345585751967612No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12220.13060411349518838No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12180.13017660412204538No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12040.12868032131604484No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12000.12825281194290186No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11880.12697028382347283No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11610.12408459555475752No Hit
CCTGCATACTGTTGGACACATCTACTTGGATTGGTACCTGCAGAAGCCAG11290.12066452056961349No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11180.11948886979347023No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11160.11927511510689871No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11140.11906136042032721No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11090.11852697370389846No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG11080.11842009636061271No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11070.11831321901732697No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10910.11660318152475492No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10730.11467938934561141No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA10720.11457251200232565No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCG10710.11446563465903989No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG10630.11361061591275388No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC10570.11296935185303937No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT10480.1120074557634676No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTACCCGTCACCC10450.11168682373361034No Hit
GACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGAATTTATTACTGCATGCAAGCTCTACAAACG10400.1111524370171816No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10390.11104555967389586No Hit
GTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAAAATCAGCAGAG10170.10869425812160931No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10080.10773236203203757No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10070.1076254846887518No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9940.10623607922603705No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9870.10548793782303677No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9850.10527418313646528No Hit
GTCGGAGACCCCGGCCGCCCGATTAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA9810.10484667376332225No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9690.10356414564389324No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9680.1034572683006075No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9610.10270912689760722No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCGGCCGGGGTCTCC9580.10238849486774997No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9540.10196098549460697No Hit
GGGCTGGAGTGGGTGGCCAACATAAAAGAAGATGGAAGTGAGAGATACTA9480.10131972143489247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)