FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700598
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT31710.4526133388904907No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29510.4212115935243892No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA28810.4112201290897205No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATT24810.3541260466058995No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20820.2971746993282881No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18910.2699122749422636No Hit
GTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCACTGGCAGTGGG18740.2674857764367012No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17320.2472173771549448No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16670.23793958875132384No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACGGGAGAAATAGTGA16480.2352276198333424No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGAAACA16080.22951821158496027No Hit
CCTCATGCATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGAATT15530.2216677752434349No Hit
CTCCTGCAGGACCAGTCAGAGTGTTAGCATCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT15140.21610110220126236No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15010.21424554452053818No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14670.20939254750941336No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13960.1992583478685352No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCAGTTATTATACTG13540.19326346920773396No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG13490.1925497931766862No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13350.19055150028975246No Hit
CTGGTATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGAATTCACT13300.1898378242587047No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13160.18783953137177098No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12940.18469935683516084No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12480.17813353734952142No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12370.17656345008121632No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTAGCAT11980.17099677703904378No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11750.16771386729622406No Hit
GTCAGAGTGTTAGCATCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGCATGGTG11440.16328907590372796No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATGCATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG11310.16143351822300378No Hit
GTGGATGCACCATGCATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGATGCTAACA11230.16029163657332735No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATACCAGTGGCCCTGGT11160.1592924901298605No Hit
GTAATAAACTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGA11070.1580078732739745No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATT10990.1568659916242981No Hit
GTGTTAGCATCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGCATGGTGCATCCA10520.15015743693244915No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10190.14544717512753388No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10190.14544717512753388No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10140.14473349909648614No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10090.14401982306543837No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCGCTCACTTTCGGCGG10070.14373435265301926No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9850.14059417811640912No Hit
CTGCTACTCTGGCTCCCAGAAACCACGGGAGAAATAGTGACGACCCAGTC9790.1397377668791518No Hit
GTCTGCGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTAGCATCAACTTAGCCTGGTA9770.13945229646673268No Hit
GTCTATTACTGTGCGAGAGCCGAGAGGGGGGGAAACAGTGGCTGGTTCGTCGAAGGCTTCTTTGACTACTGGGGC9670.13802494440463717No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9520.1358839163114939No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT9380.13388562342456017No Hit
GAGTGAATTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATACCA9350.13345741780593148No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9210.13145912491899775No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9110.13003177285690223No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9080.1296035672382736No Hit
TGCATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGAATTCACT8960.12789074476375897No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8960.12789074476375897No Hit
CTGCAGGACCAGTCAGAGTGTTAGCATCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT8890.1268915983202921No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGCATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT8890.1268915983202921No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8840.12617792228924435No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8740.1247505702271488No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8540.12189586610295777No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8410.12004030842223358No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8260.1178992803290903No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8230.11747107471046163No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC8180.11675739867941387No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACGGGAGAAATAGTGACGACCCAGTCTCCAGCCACC8000.11418816496764192No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACGGGAGAAATAGTGACGACCCAGTCTC7980.11390269455522284No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7920.11304628331796551No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7790.11119072563724132No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7600.10847875671925983No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7580.10819328630684072No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7460.1064804638323261No Hit
GAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTAGCATCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG7440.10619499341990699No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGAATTCACTCTCACCATCAGCAG7370.10519584697644012No Hit
TGCATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGATGCTAACACTCTGACTGGTC7360.10505311177023058No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7300.10419670053297325No Hit
CAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGT7230.10319755408950639No Hit
ATGCATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGATGCTAACACTCTGACTGGT7230.10319755408950639No Hit
CTCCCAGGCTCCTCATGCATGGTGCATCCACCAGGGCCACTGGTATCCCA7210.1029120836770873No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7190.1026266132646682No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7150.10205567243982998No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACGGGAGAAATAGTGACGACC7150.10205567243982998No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7060.100771055583944No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGATGCTAACACTCTGACTGGTCCTGCAGGAGAGGGTGGCTCTTTCCC7060.100771055583944No Hit
CACCATCAGCAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGGCCTCCGCTCA7040.1004855851715249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1100.053.1422231
GGTATCA5350.047.5621451
GTATCAA14900.043.617321
TATCAAC16600.039.14492
ATCAACG17550.037.0259483
AACGCAG17950.036.3923956
CAACGCA18050.036.1907775
TCAACGC18050.035.809824
CGCAGAG19300.034.0249568
AGAGTAC19600.033.5041611
GCAGAGT19850.033.0821959
CGATCAG652.3536399E-531.77962329
CAGAGTA22000.029.84916510
TATTAGA703.9405197E-529.4696312
CGTATGC605.312419E-428.84869441
TCGTATG605.3258403E-428.8362640
AGTACGG19150.028.36676413
GAGTACG19300.028.14629712
GAGCAAA7850.027.73275870
GGATAGC756.300377E-527.5089151
Produced by FastQC (version 0.11.5)