FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005578408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700598
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT31950.45603898383951996No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30370.4334868212584107No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA28850.41179106991455866No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATT26130.3729670938255604No Hit
GTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCACTGGCAGTGGG17810.25421140225921285No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17740.25321225581574597No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16840.24036608725688624No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16470.2350848846271328No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACGGGAGAAATAGTGA16140.23037462282221757No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGAAACA15270.2179566598819865No Hit
CCTCATGCATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGAATT14290.20396860967345037No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13910.1985446718374874No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13870.19797373101264917No Hit
CTCCTGCAGGACCAGTCAGAGTGTTAGCATCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT13440.19183611714563845No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13260.1892668834338665No Hit
CTGGTATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGAATTCACT12940.18469935683516084No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12780.182415593535808No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG12530.17884721338056916No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12410.17713439090605454No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCAGTTATTATACTG11800.16842754332727186No Hit
GTGGATGCACCATGCATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGATGCTAACA11530.16457369275961392No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11280.16100531260437512No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11150.15914975492365094No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTAGCAT11110.15857881409881272No Hit
GTCAGAGTGTTAGCATCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGCATGGTG11020.15729419724292676No Hit
CTGCTACTCTGGCTCCCAGAAACCACGGGAGAAATAGTGACGACCCAGTC10970.156580521211879No Hit
GTAATAAACTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGA10770.15372581708768793No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10560.15072837775728734No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10510.15001470172623957No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10420.1487300848703536No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10320.1473027328082581No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCGCTCACTTTCGGCGG10210.145732645539953No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10050.14344888224060015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10010.14287794141576196No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9990.14259247100334285No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9870.14087964852882823No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATGCATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG9790.1397377668791518No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATACCAGTGGCCCTGGT9770.13945229646673268No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9760.13930956126052316No Hit
GTGTTAGCATCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGCATGGTGCATCCA9690.13831041481705628No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9690.13831041481705628No Hit
GTCTATTACTGTGCGAGAGCCGAGAGGGGGGGAAACAGTGGCTGGTTCGTCGAAGGCTTCTTTGACTACTGGGGC9630.13745400357979895No Hit
GTCTGCGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTAGCATCAACTTAGCCTGGTA9560.1364548571363321No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9480.1353129754866557No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9470.13517024028044614No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9350.13345741780593148No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATT9290.13260100656867418No Hit
CTGCAGGACCAGTCAGAGTGTTAGCATCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT8910.1271770687327112No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8900.12703433352650165No Hit
GAGTGAATTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATACCA8890.1268915983202921No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACGGGAGAAATAGTGACGACCCAGTCTCCAGCCACC8460.12075398445328134No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT8440.12046851404086223No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC8380.11961210280360493No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8380.11961210280360493No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8320.1187556915663476No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGCATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT8220.11732833950425209No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8080.11533004661731835No Hit
TGCATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGAATTCACT7980.11390269455522284No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACGGGAGAAATAGTGACGACCCAGTCTC7960.11361722414280373No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7940.11333175373038462No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7550.10776508068821207No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7420.1059095230074879No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7380.10533858218264969No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7230.10319755408950639No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7170.10234114285224909No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7100.10134199640878222No Hit
CAGTAATAAACTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGT7030.10034284996531535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)