FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626288
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG704.2018655E-1044.58397340
CTCGTAT704.2564352E-1044.54082539
GGTATCA7550.039.9882281
GTATCAA18350.039.2626531
GTTTCGT400.002671590534.29712711
TCAACGC21400.033.3381844
ATCAACG21400.033.1779063
TATCAAC21650.032.955852
CGTATGC958.283678E-932.88054741
CAACGCA21850.032.6515855
AACGCAG22750.031.3573726
ACGCAGA23900.029.9920467
AGAGTAC24800.028.48874311
CGCAGAG25100.028.4215248
AGTACGG20900.028.38948613
CAGAGTA25150.028.09227810
GAGTACG21400.027.72618312
CGTCTAT500.00795492127.4398923
TTTAACG2150.027.26669134
TAACGGC2050.026.97741536
Produced by FastQC (version 0.11.5)