FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405795
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA52030.3701108625368564No Hit
CTCCAGCCCCTTCCCTGGAGACTGGCGGACCCAGGTCATCCAATAGGTAC32060.22805601101156284No Hit
GTATATTACTGTGCGAGAGATTTCTTTGATCCTAGTGGTCTGAGAACAGA30450.21660341657211757No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30150.21446939276352525No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28950.20593329752915612No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG26220.18651368087096626No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25330.18018274357214245No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23880.1698682951639464No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23840.16958375865613406No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG22560.16047859040614032No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22520.160194053898328No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22490.15998065151746876No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC22130.157419822947158No Hit
CCTTTAGTACCTATTGGATGACCTGGGTCCGCCAGTCTCCAGGGAAGGGG20020.1424105221600589No Hit
GGGCTGGAGTGCGTGGCCAACATAAATCAAGATGGAAGTGAGACATACTA19570.13920948644717046No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC19010.1352259753377982No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18960.13487030470303282No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18310.13024658645108284No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC18260.12989091581631745No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17740.12619194121475746No Hit
CTATGTGGACTCTGTCAAGGGCCGATTCACCGTCTCCAGAGACAACGGCAAGAACTCTCTGTATCTGCAAATGAA17600.12519606343741443No Hit
GTCAAGGGCCGATTCACCGTCTCCAGAGACAACGGCAAGAACTCTCTGTA16980.12078574756632368No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16890.12014554042374598No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16680.11865172375773139No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC16490.11730017534562294No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16180.11509501741007756No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15820.11253418883976682No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA15780.11224965233195452No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15700.11168057931632991No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTTGAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT15610.11104037217375222No Hit
GAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGT15320.108977482492113No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15290.10876408011125378No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15180.10798160471476993No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGACACTTGGTCCAGCCTGGGGGGTCCCTGAGAGTCTCCTGTGCAGC15080.10727026344523917No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15080.10727026344523917No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14590.10378469122453843No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCGGGT14350.1020774721776646No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14350.1020774721776646No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG14340.10200633805071152No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA14260.1014372650350869No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14230.10122386265422767No Hit
GTCTCCAGCCTCCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCTTTCAGAGTGTGAGGCG14140.10058365551164998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1250.044.24856641
GTATCAA22850.043.692661
TCGTATG1350.040.9488840
GGTATCA8150.040.4124641
TATCAAC25700.038.980862
TCAACGC26700.037.775214
CGAGACG1550.037.64072823
ATCAACG27000.037.2284243
CAACGCA27500.036.6762925
AACGCAG28500.035.388156
CTTATAC1600.034.30851
TTATACA1950.033.428792
AGAGTAC30250.033.340911
CGCAGAG30850.032.6924558
TATACAC1900.032.5004733
TCTGGAG21200.032.3087470
CTCGTAT1750.031.57104139
GCAGAGT32000.031.5175699
TATGCCG1800.030.77008243
GTTAGCC902.0968218E-730.4942743
Produced by FastQC (version 0.11.5)