FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405795
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA45910.3265767768415736No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33060.2351694237068705No Hit
CTCCAGCCCCTTCCCTGGAGACTGGCGGACCCAGGTCATCCAATAGGTAC32480.23104364434359206No Hit
GTATATTACTGTGCGAGAGATTTCTTTGATCCTAGTGGTCTGAGAACAGA29230.2079250530838422No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26140.18594460785534164No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24950.17747964674792555No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG24060.17114870944910174No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22500.16005178564442182No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22070.15699301818543954No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC21070.1498796054901319No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20550.14618063088857194No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20390.1450424848573227No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG19940.14184144914443428No Hit
GGGCTGGAGTGCGTGGCCAACATAAATCAAGATGGAAGTGAGACATACTA19710.14020536422451352No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC19690.1400630959706074No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19230.13679092613076588No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18040.12832596502334978No Hit
CCTTTAGTACCTATTGGATGACCTGGGTCCGCCAGTCTCCAGGGAAGGGG17980.1278991602616313No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC17800.12661874597647593No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC17190.1222795642323383No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16960.12064347931241752No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16920.12035894280460523No Hit
CTATGTGGACTCTGTCAAGGGCCGATTCACCGTCTCCAGAGACAACGGCAAGAACTCTCTGTATCTGCAAATGAA16260.11566409042570219No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16200.11523728566398372No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16090.11445481026749989No Hit
GAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGT15690.11160944518937682No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15300.10883521423820684No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15260.10855067773039453No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA14960.10641665392180225No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGACACTTGGTCCAGCCTGGGGGGTCCCTGAGAGTCTCCTGTGCAGC14950.10634551979484919No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA14800.10527850789055304No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14670.10435376424016303No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTTGAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT14230.10122386265422767No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14200.10101046027336845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAG200.00718212452.07003846
GTATCAA23300.040.9161951
GGTATCA10050.036.1698071
TATCAAC26450.036.142142
TCAACGC27850.034.2010544
ATCAACG28050.033.9511573
CAACGCA28350.033.5978625
AACGCAG28850.033.133166
AGAGTAC30800.031.37250711
CGCAGAG31000.030.9457348
TTTCGCG450.004694299330.57090633
TCTGGAG21300.030.47099170
GCAGAGT32950.029.218339
AGTACGG30450.027.2329313
CAGAGTA35750.027.2164410
GAGTACG30650.027.16702312
ACGCAGA35950.026.6847787
TACGGGA14550.024.73084415
ATTTGCA17550.024.17911138
TTAATCG1001.5759177E-524.12628637
Produced by FastQC (version 0.11.5)