FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005578414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719429
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22650.3148330134036854No Hit
GTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCT22170.3081610555037398No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19820.2754962616185892No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGAAACA17630.24505545370008713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16780.2332405282522667No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15730.2186456203461356No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15300.21266865806076765No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14810.20585770103790646No Hit
CCATCAACACTCTGCAACCTGGAGATTTTGCAACTTACTACTGTCAACAG14690.20418971156292No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14070.19557176594215692No Hit
GTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGGGCATTAGCAACTATTTAAATTGGTATCAGCAGAAA13790.19167979050052195No Hit
GAGTTCACTCTCACCATCAACACTCTGCAACCTGGAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTGGC13710.19056779751719768No Hit
GTGTATTACTGTGCGAAGGCGGGGGTCGTTCGAGGTTCGGGGAGTTATTGGTCAAGGAACGCTGGTGCGAATTA13680.19015080014845107No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13560.18848281067346465No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTC13490.18750981681305592No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13350.18556382909223842No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGGGCATTAGCAA13210.18361784137142095No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12920.17958686680687044No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12690.17638988697981317No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT12650.175833890488151No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCTCA12460.17319290715275587No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12180.1693009317111209No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12140.16874493521945877No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11900.16540895626948593No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11860.1648529597778238No Hit
GATCTATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCA11610.16137798170493545No Hit
CTCCAGCCCCGTCCCTGGAGCCTGGCGGACCCAGCCCATCCAATAGTTTC11490.15970999222994903No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11240.15623501415706068No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT11220.1559570159112296No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10990.1527600360841723No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTCCAAAGGCCA10900.1515090439779325No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10850.15081404836335482No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCTCACTTTGCAAAGT10750.14942405713419948No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10010.13913812203844994No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9960.13844312642387227No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9920.1378871299322101No Hit
GTAGTAAGTTGCAAAATCTCCAGGTTGCAGAGTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCA9740.1353851457197305No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9670.13441215185932176No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9190.1277401939593761No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9120.12676720009896736No Hit
ACTTACTACTGTCAACAGAGTTTCAGTGGCCTTTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAA9110.12662820097605185No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT9060.12593320536147418No Hit
CTCCTGATCTATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTT9060.12593320536147418No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC8970.12468221325523435No Hit
GGGCTGGAGTGGGTGGCCAACATAAAACAAGATGGAAGTGAGAAACACTA8900.12370921939482563No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8790.12218022904275473No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8720.121207235182346No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8680.12065123869068387No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8660.1203732404448528No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8570.11912224833861297No Hit
GTCTGCATCTGTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGG8560.11898324921569745No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8450.11745425886362658No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8360.11620326675738676No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8300.11536927201989357No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8150.11328428517616054No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8130.11300628693032948No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8120.11286728780741392No Hit
GTCCAAAGGCCACTGAAACTCTGTTGACAGTAGTAAGTTGCAAAATCTCC7960.11064330184076539No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC7810.10855831499703236No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA7810.10855831499703236No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7770.10800231850537022No Hit
ATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACTCTCA7620.1059173316616372No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7410.102998350080411No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAACACTCTGCAACCTGGAGATT7230.10049636586793138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)