FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005578417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972749
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGTCCACCGATGGCCAGGGAGG58100.597276378593039No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCATTATGTCTTCGGAACTGGGACCAAAGTCACCGTC42690.43885935631905043No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT39010.40102842562675467No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA38530.3960939564060205No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC32970.3389363545991823No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32490.3340018853784481No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29540.3036754599593523No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT27130.27890031241358254No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA25560.2627604860040977No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25260.25967644274113877No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25220.2592652369727443No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG24910.25607839226768675No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC24270.24949909997337444No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC23700.24363941777375253No Hit
CCCCTGAGGGCCGCTGATTATTGCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAATAAA22530.23161164904821285No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAATGACCA21920.22534076108019643No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21770.22379873944871698No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21400.2199950860910677No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20710.21290178658626224No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20150.20714490582873896No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20100.2066308986182458No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19500.20046281209232802No Hit
GACGTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAATGACCACTC18950.1948087327769034No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17880.18380897847235No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCGGTGGACTCCGGT17850.1835005741460541No Hit
GCATGGGATGACAGCCTGAGTGGTCATTATGTCTTCGGAACTGGGACCAA17240.17722968617803772No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16390.16849156359965417No Hit
CTCTTGTTCTGGAAGCAGGTCCAACATCGGAACGAACTATGTTTATTGGTACCAGCAACTCCCAGGAACGGCCCC16300.16756635062076652No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16260.16715514485237198No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16140.16592152754718845No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGGTCCAACATCGGAACGAA16000.16448230735780764No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15840.16283748428422953No Hit
ACGTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAATGACCACTCA15790.1623234770737364No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15640.16078145544225694No Hit
ATGTTTATTGGTACCAGCAACTCCCAGGAACGGCCCCCAAACTCCTCATC15340.15769741217929806No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCGGTGGACTCCGGTCCGAGGATG14710.15122092132708437No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14630.15039850979029534No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14090.14484723191696935No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAATAAACATAGTTCGTT13970.1436136146117858No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13650.1403239684646296No Hit
CATAATGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC13560.13939875548574196No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13490.13867914539105153No Hit
GCCTTGGGCTGACGTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATA13480.1385763439489529No Hit
GATTATTGCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGG13340.1371371237595721No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC13200.1356979035701913No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13000.1336418747282187No Hit
GTCATTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTACGTCAGCCC12840.1319970516546406No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTCATTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTACGTCAGC12790.13148304444414746No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGGTCCAACATCGGAACGAACTAT12710.1306606329073584No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCACCGATG12570.1292214127179776No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCAC12520.12870740550748447No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12480.12829619973908996No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12290.12634297233921596No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12250.12593176657082145No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12250.12593176657082145No Hit
GGATGACAGCCTGAGTGGTCATTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTACGTCAGCCCAAGGCCAA12230.1257261636866242No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG12060.12397853917094749No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT12050.12387573772884887No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11870.12202531177107352No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG11670.11996928292910093No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11560.118838467066016No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG11470.11791325408712834No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11450.11770765120293107No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG11430.11750204831873381No Hit
GATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAATAAACAT11150.11462360793997217No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT11120.1143152036136763No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT11000.11308158630849274No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10990.11297878486639411No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10950.1125675790979996No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10920.1122591747717037No Hit
ATGTTGGACCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGT10910.11215637332960507No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10780.11081995458232288No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATTGCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTA10770.11071715314022425No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT10630.10927793295084343No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC10630.10927793295084343No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10590.10886672718244891No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10570.10866112429825166No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGCAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG10450.1074275069930681No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG10430.10722190410887084No Hit
TCGGTGGACTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGG10360.10650229401418043No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG10330.10619388968788454No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG10290.10578268391949001No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGGT10290.10578268391949001No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT10160.10444626517220783No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG10070.10352105219332017No Hit
ATCCATGGTGGGTCCTTCAGTGATTACTACTGGAGCTGGATCCGCCAGTC10040.10321264786702428No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10040.10321264786702428No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10030.10310984642492566No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9990.10269864065653113No Hit
GCCCCCAAACTCCTCATCTATAGCAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT9970.10249303777233387No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG9920.10197903056184071No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG9910.10187622911974209No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC9910.10187622911974209No Hit
GTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTC9770.10043700893036128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)