FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972749
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGTCCACCGATGGCCAGGGAGG49130.505063485030568No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCATTATGTCTTCGGAACTGGGACCAAAGTCACCGTC41040.4218971183727765No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT36100.37111320597605346No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34710.3568238055243439No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33140.34068397911485904No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC31940.32834780606302344No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA27860.2864048176867825No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG27570.2834235758659222No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27200.27961992250827294No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG26960.2771526878979058No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT24080.24754587257350044No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC23550.24209739614227307No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23400.24055537451079362No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC23180.23829374278462379No Hit
CCCCTGAGGGCCGCTGATTATTGCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAATAAA22140.22760239280636632No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAATGACCA21210.21804185869119375No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20710.21290178658626224No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19460.2000516063239335No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19290.1983039818082568No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19100.19635075440838284No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18440.1895658592298733No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16990.17465965012557197No Hit
CTCTTGTTCTGGAAGCAGGTCCAACATCGGAACGAACTATGTTTATTGGTACCAGCAACTCCCAGGAACGGCCCC16730.1719868126310076No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16410.16869716648385144No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCGGTGGACTCCGGT16360.16818315927335828No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16160.1661271304313857No Hit
GACGTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAATGACCACTC16090.16540752033669529No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15370.15800581650559395No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15350.15780021362139668No Hit
GCATGGGATGACAGCCTGAGTGGTCATTATGTCTTCGGAACTGGGACCAA15350.15780021362139668No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCGGTGGACTCCGGTCCGAGGATG15180.15605258910571998No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15170.15594978766362136No Hit
ATGTTTATTGGTACCAGCAACTCCCAGGAACGGCCCCCAAACTCCTCATC15160.1558469862215227No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC14950.1536881559374515No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGGTCCAACATCGGAACGAA14830.15245453863226793No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14290.14690326075894192No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAATAAACATAGTTCGTT14100.14495003335906798No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13880.14268840163289812No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13650.1403239684646296No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG13560.13939875548574196No Hit
ACGTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAATGACCACTCA13410.1378567338542625No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGGTCCAACATCGGAACGAACTAT13170.1353894992438954No Hit
GATTATTGCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGG13080.13446428626500773No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12750.13107183867575295No Hit
GCCTTGGGCTGACGTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATA12500.1285018026232872No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG12500.1285018026232872No Hit
GTCATTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTACGTCAGCCC12440.12788499397069542No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12400.1274737882023009No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG12310.12654857522341323No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12030.1236701348446516No Hit
CATAATGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC12010.12346453196045434No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT12010.12346453196045434No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11970.12305332619205983No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTCATTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTACGTCAGC11860.12192251032897489No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG11850.12181970888687627No Hit
GGATGACAGCCTGAGTGGTCATTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTACGTCAGCCCAAGGCCAA11770.12099729735008723No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11730.1205860915816927No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCAC11540.11863286418181876No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCACCGATG11480.11801605552922696No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG11400.11719364399243792No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11350.11667963678194478No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11240.11554882091885986No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT11150.11462360793997217No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC11100.11410960072947902No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11060.11369839496108451No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10810.11112835890861876No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG10810.11112835890861876No Hit
GATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAATAAACAT10710.11010034448763248No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGGT10660.10958633727713933No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10660.10958633727713933No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT10580.10876392574035029No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10550.10845552141405439No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10220.1050630738247996No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG10180.10465186805640511No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG10160.10444626517220783No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG10080.10362385363541879No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT10040.10321264786702428No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10000.10280144209862975No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC9940.10218463344603798No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9900.10177342767764347No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC9870.10146502335134756No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT9820.10095101614085442No Hit
ATGTTGGACCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGT9810.1008482146987558No Hit
TCGGTGGACTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGG9770.10043700893036128No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC9740.10012860460406538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3000.043.3484652
GTGGTAT3200.041.7580381
GTATCAA19250.041.471581
GGTATCA7650.041.205181
TATCAAC21300.037.435852
TACGGGT952.401066E-1036.0236515
ATCAACG22750.034.738253
TCAACGC22700.034.6640554
CAACGCA23400.033.776775
AACGCAG23550.033.559916
CGCAGAG24500.032.2569478
CAGAGTA25000.031.74702510
AGAGTAC24800.031.73531711
CGTAAAC553.0846518E-431.65512550
AGTATTC450.004514765530.81460646
GCAGAGT26300.030.177789
ACGCAGA26300.030.0492487
CTCGCTA704.0514307E-529.35166423
AAGTATT605.2903057E-428.87064445
TCGGGAT605.665157E-428.53487222
Produced by FastQC (version 0.11.5)