FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005578420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119500
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGGAGGCTGCCCTGGTTTTTGTTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGTGGTATAAAACACT65440.5845466726217061No Hit
CTCCTAAACTGCTCATTTACTGGGCGTCTACCCGGGAATCCGGGGTCCCT53700.4796784278695846No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50580.4518088432335864No Hit
GTTTTATACCACTCCAACAATAAGAACTACTTGGCTTGGTACCAACAAAA42470.3793657882983475No Hit
ATTTTATACTACTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT35470.3168378740509156No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32890.2937918713711478No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32710.292184010719071No Hit
GTGCTGATGGTGAGACTGAAATCTGCCCCAGACCCGCTGCCACTGAATCG32250.2880750334970969No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30850.2755694506476105No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29400.262617239839214No Hit
GTAATAAACCGCCACATCGTCAGCCTGCAGGGTGCTGATGGTGAGACTGAAATCTGCCCCAGACCCGCTGCCA29180.2606520768200089No Hit
GTATAAAACACTCTGGCTGGAGTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA29060.25958016971862435No Hit
GTATAAAATTGGTGACAGTAATAAACCGCCACATCGTCAGCCTGCAGGGTGCTGATGGTGAGACTGAAATCTGCC27950.24966502903081733No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26480.23653416703885663No Hit
GTTTATTACTGTCACCAATTTTATACTACTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA25470.22751228226887002No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG24850.22197409557838324No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24340.21741849039749886No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGTTCTCTGGTGC24300.2170611880303707No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23600.2108083966056275No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC23480.20973648950424295No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22280.1990174184903975No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21290.190174184903975No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21190.18928092898615453No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20540.18347476552032158No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20490.18302813756141134No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19960.17829388119696293No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAACTCCAGCCAGAGTGTTTTATA19700.17597141581062975No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19280.17221974095578382No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCAGTCTCAC19260.17204108977221974No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18930.16909334524341224No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18790.1678427869584636No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18610.16623492630638678No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18020.1609647163912461No Hit
GTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGTAAATGAGCAGTTTAGGAGGCTGCCCTGGTTTTTGTTGGTA17830.15926753014738723No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17550.15676641357748994No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17550.15676641357748994No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16700.14917373827601607No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16500.14738722644037516No Hit
ATTCAGTGGCAGCGGGTCTGGGGCAGATTTCAGTCTCACCATCAGCACCC16250.14515408664582402No Hit
CCTTGGTCCCTTGGCCGAACGTCCAGGGAGTAGTATAAAATTGGTGACAGTAATAAACCGCCACATCGTCAGC16120.14399285395265743No Hit
CCATCAGCACCCTGCAGGCTGACGATGTGGCGGTTTATTACTGTCACCAA15670.13997320232246538No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15610.1394372487717731No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15590.13925859758820902No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15590.13925859758820902No Hit
ATACTACTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGA15460.13809736489504243No Hit
GGTTCTCTGGTGCCTATGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG15410.1376507369361322No Hit
GTAAATGAGCAGTTTAGGAGGCTGCCCTGGTTTTTGTTGGTACCAAGCCA15300.1366681554265297No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15260.13631085305940152No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14830.13246985261277355No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14770.13193389906208128No Hit
CAGTAATAAACCGCCACATCGTCAGCCTGCAGGGTGCTGATGGTGAGACTGAAATCTGCCCCAGACCCGCTGCCA14700.131308619919607No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14350.12818222420723535No Hit
CATCAGCACCCTGCAGGCTGACGATGTGGCGGTTTATTACTGTCACCAATTTTATACTACTCCCTGGACGTTCGG14260.12737829388119695No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAACTCCAGCCAGAGTGTTTTATACCACTCCAA14210.12693166592228675No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14170.12657436355515855No Hit
GAGTAGTATAAAATTGGTGACAGTAATAAACCGCCACATCGTCAGCCTGCAGGGTGCTGATGGTGAGACTGAAAT14130.1262170611880304No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCATAGAGGTCATCTTCAGGGAGA14070.12568110763733809No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGTAAATGAGCA13900.12416257257704333No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGTTCTCTGGTGCCTATGGGGACATCGTGATGA13850.12371594461813308No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13650.12192943278249219No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13380.11951764180437696No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13120.11719517641804378No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13080.11683787405091559No Hit
CTATGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG13060.11665922286735149No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAACTCCAGCCAGAGTGTTTTATACCACTCCAACAATAAGAACTA12690.11335417597141582No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12680.11326485037963377No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12650.11299687360428762No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12400.11076373380973649No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12390.11067440821795445No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12300.10987047789191604No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12070.10781598928092898No Hit
GCTCATTTACTGGGCGTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTT12050.10763733809736489No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12050.10763733809736489No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12020.10736936132201877No Hit
GTCCAGGGAGTAGTATAAAATTGGTGACAGTAATAAACCGCCACATCGTCAGCCTGCAGGGTGCTGATGGTGAGA12020.10736936132201877No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11920.10647610540419832No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG11890.10620812862885216No Hit
TCGTCAGCCTGCAGGGTGCTGATGGTGAGACTGAAATCTGCCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC11880.10611880303707012No Hit
GGTCTGGGGCAGATTTCAGTCTCACCATCAGCACCCTGCAGGCTGACGAT11870.10602947744528808No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTCCAGGGAGTAGT11860.10594015185350604No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAACTCCAGC11850.10585082626172398No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11530.10299240732469853No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11400.10183117463153192No Hit
TTATTACTGTCACCAATTTTATACTACTCCCTGGACGTTCGGCCAAGGGA11240.10040196516301919No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11220.10022331397945511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)