FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560437
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG14720.26265218035211807No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAACTTCCAGGAAGAGC14230.2539090031528968No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCCGAATCA11180.19948718589243752No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC11170.1993087537046983No Hit
GTTTATTACTGTGCGAGATTACCGAAACCAGTGGCTGTCGGGCACTTCGA11040.19698913526408857No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGAGTTGAGCTTCAGGGAGA11040.19698913526408857No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10870.19395578807252198No Hit
GGTTATGATGTACACTGGTACCAGCAACTTCCAGGAAGAGCCCCCAAACTCCTCATCTTTGATAGTAGCAATCGG10560.18842439025260646No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTGCTA9950.1775400268005146No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9790.17468511179668725No Hit
GGCTACTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGG9720.17343608648251277No Hit
GTCCTATGACAGTAGCCTGAGTGATTCGGATTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCC9680.1727223577315559No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9240.16487134147103064No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA8940.1595183758388543No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAAGAGCCCCCAAACTCCTCAT8890.15862621490015827No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8810.1571987573982446No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8810.1571987573982446No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT8810.1571987573982446No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG8620.15380854583119957No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8590.15327324926798194No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8490.15148892739058983No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8450.15077519863963298No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT8430.15041833426415457No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8000.1427457501913685No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7930.14149672487719403No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7630.13614375924501773No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7620.13596532705727854No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7560.13489473393084325No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7400.13203981892701588No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG7270.12972020048640615No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7250.12936333611092773No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG7220.12882803954771008No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7190.12829274298449245No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7160.12775744642127482No Hit
ACAGTAGCCTGAGTGATTCGGATTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTG7130.12722214985805722No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG7120.12704371767031797No Hit
GATTATTACTGCCAGTCCTATGACAGTAGCCTGAGTGATTCGGATTGGGT7100.12668685329483956No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6980.12454566704196902No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC6810.12151231985040245No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC6780.12097702328718483No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC6630.11830054047109666No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6560.11705151515692218No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT6460.11526719327953007No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG6450.11508876109179086No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6450.11508876109179086No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6290.11223384608796351No Hit
GTCTCTGGTGGCTCCATCAGAAGTTATAGTTACTATTGGGCCTGGATCCG6170.11009265983509296No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6140.10955736327187533No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC6010.10723774483126561No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6010.10723774483126561No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC5990.10688088045578718No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA5980.10670244826804798No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC5960.10634558389256954No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT5950.10616715170483033No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT5900.10527499076613428No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA5900.10527499076613428No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC5830.1040259654519598No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5820.1038475332642206No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5790.10331223670100297No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5700.10170634701135008No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC5700.10170634701135008No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5680.10134948263587165No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGTAGCCTGAGTGATTCGGATTGGGTGTTCGGCGGAGGGAC5650.10081418607265401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATGT200.00727351.90060842
GTTATAA200.00738479751.7007736
ACGGGTA200.00748993851.5162615
AATCGAG554.830636E-448.55163670
GTCCGTC305.113444E-448.00218267
AGCGTCA652.237357E-1047.553473
GGTATCA4150.043.0413441
ATATACC503.9606E-641.213014
TATACCA503.9606E-641.213015
GTATCAA9700.041.0787121
ATCTAAA350.001376334339.2574921
CGACACG350.001376938339.25398621
CATATAT451.0268678E-438.1670071
TACGGAG3650.036.5870
TATCAAC10950.035.4482572
GTACAAT400.002651135434.3503041
TGGTATC1400.034.3503042
ATCAACG11300.034.3441773
TATATAC501.9103498E-434.3441733
TCAACGC11350.033.8902854
Produced by FastQC (version 0.11.5)