FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723880
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13620.18815273249709896No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA11900.16439188815825828No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11430.15789909929822624No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA10190.14076918826324805No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9390.12971763275681053No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9070.12529701055423553No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8710.12032381057633862No Hit
GTATATTACTGTGCGCGAGATAATCCCTTAGTGGGATTTGACTTCTGGGGCCAGGGAGCCCTGGTCACCGTCTC8540.11797535503122064No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8270.11424545504779798No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT8190.11314029949715423No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8140.1124495772780019No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8080.11162071061501906No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGTGGCCCTGTT7600.10498977731115654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.043.457871
GTATCAA20250.042.4821851
TATCAAC23100.037.363412
TCAACGC23550.036.496464
ATCAACG23550.036.4914173
CAACGCA24050.035.8850365
AACGCAG24550.035.1541826
AGAGTAC25200.034.38570811
TTACTAG501.9444122E-434.2426954
CGCAGAG25950.033.389598
ACGCAGA26800.032.20287
GCAGAGT27300.031.613019
CAGAGTA27900.031.18081310
CGAACCT1251.05501385E-1030.78217553
ACGGGTT450.00478583930.44846716
GAGTACG26150.028.81435812
AGTACGG26300.028.65001713
ACGAACC1404.0199666E-1027.4569652
CCCGCGT500.0079614327.43585624
TGACTCG500.0080126327.39983610
Produced by FastQC (version 0.11.5)