FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944755
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTGACTACTGGAGCTGGATCCGCCAGTC45540.48202973257617054No Hit
GTAATAAACAGCCGTATCAGCGGCGGTCACAGAGGTCAGGTTCAGGGAGA28830.305158480240909No Hit
GTATAATGACTGGCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCACACGAACTGTGGCTGCACCAT23460.2483183470846939No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19750.20904890685945035No Hit
GTATTAGCACTTTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCA19480.20619102306947304No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19450.20587348042614223No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19280.20407407211393433No Hit
GTTTATTACTGTGCGAGAGGACTTATTGTGGTGGTCGCTGCTCCCTTCTT18650.19740567660398728No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA17390.18406888558409323No Hit
GTCATTATACTGTTGACAGTAATAAATTGCAAAATCTTCAGACTGCAGGCTGCGGATGGTGAGAGTGAACT16370.17327243571084566No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16050.1698853141819837No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15110.15993564469095162No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14150.1497742801043657No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC14110.14935088991325793No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13130.13897783023111812No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12790.13537901360670226No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12650.13389714793782515No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11810.12500595392456246No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11680.12362993580346228No Hit
CATACAACCCGTCCCTCAAGAGTCGCGTTAGCATATCATTAGACACGTCCAAGAACCAGTTCTCCCTGAACCTGA11680.12362993580346228No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11650.12331239316013146No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT11390.12056035691793111No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11350.12013696672682336No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11270.11929018634460786No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11210.11865510105794623No Hit
GTCTGGGACAGAGTTCACTCTCACCATCCGCAGCCTGCAGTCTGAAGATT10530.11145746780911454No Hit
TTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCATCCACCAGGGCCA10490.1110340776180068No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10480.11092823007022984No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10430.11039899233134516No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10310.10912882175802192No Hit
GGGTTCCCTGGCCCCAGGAGTCAAAGAAGGGAGCAGCGACCACCACAATAAGTCCTCTCGCACAGTAATAAACAG10210.10807034628025255No Hit
CAATAAGTCCTCTCGCACAGTAATAAACAGCCGTATCAGCGGCGGTCACA10030.10616509042026769No Hit
TCATTAGACACGTCCAAGAACCAGTTCTCCCTGAACCTGACCTCTGTGACCGCCGCTGATACGGCTGTTTATTA9830.10404813946472895No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9800.10373059682139812No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9720.10288381643918265No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9660.10224873115252102No Hit
CTCCTGCAGGGCCAGTCACAGTATTAGCACTTTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT9600.10161364586585941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2000.046.3923841
TGGATTG15950.044.3637970
GTATCAA19450.042.050421
GGTATCA7800.041.854481
TAATGAT10250.039.44822370
TATCAAC22200.036.9688452
TCAACGC23300.034.6321874
CAACGCA23650.034.264855
ATCAACG23750.034.118773
AACGCAG23950.033.6922726
ACGGGAC1056.7484507E-1032.71098316
CGCAGAG25050.031.938628
CACGATT450.00414406931.35875964
AGAGTAC25550.031.31525611
GCAGAGT26050.030.5807579
GTAATAT450.004729079530.52378714
ATGATAG450.004730304230.5221735
TAACGGC802.7747246E-630.16822436
TGTTAGA4050.029.95142670
ACGCAGA27400.029.3246787
Produced by FastQC (version 0.11.5)