FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005578429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901570
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30550.33885333362911363No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24070.2669787149084375No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23180.2571070465964928No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22840.25333584746608695No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20010.22194616058653238No Hit
CTCCTGATCTCTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT19290.21396009183979062No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAACTATGTAAACTGGTATCAGCAGAAA18410.20419934114932842No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17870.19820978958927207No Hit
GATCAGGAGAGTAGGGGCTTTCCCCGGTTTCTGCTGATACCAGTTTACAT17810.1975442838603769No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17810.1975442838603769No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17440.19344033186552348No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16940.18789445079139724No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16700.18523242787581662No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16470.18268132258171854No Hit
GAGTAAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA16410.1820158168528234No Hit
GATTTTACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTTCTACTGTCAACAGACTCACAGTACC16380.18168306398837583No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16050.17802278247945252No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15820.17547167718535442No Hit
GGGTACAACCAGCTGCTATGTGATGGTCCGGCCTTGCGCCTTTGATTTCTGGGGCCAAGGGACAATGGTCACCGT15800.1752498419423894No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15650.1735860776201515No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15600.1730314895127389No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15580.17280965426977385No Hit
AGGTAGAACTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15090.16737469081713013No Hit
GTGCAGCCACAGTTCGTTTGAGTTCTACCTTGGTCCCTTGGCCGAACGTC15050.1669310203312No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14920.1654890912519272No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCTACCTTGGTCCCTTGGCCGAACGTCCTGAGGGTA14880.1650454207659971No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14820.16437991503710195No Hit
GGGTACTGTGAGTCTGTTGACAGTAGAAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTAAAAT14720.16327073882227672No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14400.1597213749348359No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14280.1583903634770456No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13550.15029337710882126No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13290.14740951895027563No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13190.1463003427354504No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12940.14352740219838728No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12860.14264006122652706No Hit
ATGTAAACTGGTATCAGCAGAAACCGGGGAAAGCCCCTACTCTCCTGATC12650.14031079117539402No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12450.13809243874574353No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12330.13676142728795324No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12170.13498674534423286No Hit
GAGTAGGGGCTTTCCCCGGTTTCTGCTGATACCAGTTTACATAGTTGCTA12130.13454307485830275No Hit
GTTCTACCTTGGTCCCTTGGCCGAACGTCCTGAGGGTACTGTGAGTCTGTTGACAGTAGAAAGTTGCAGAATCTT11940.13243564005013475No Hit
ACCTTGGTCCCTTGGCCGAACGTCCTGAGGGTACTGTGAGTCTGTTGACA11810.13099371097086193No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11780.13066095810641437No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCGTCC11620.12888627616269396No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGG11590.1285535232982464No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC11490.12744434708342114No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11380.12622425324711337No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11310.1254478298967357No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11310.1254478298967357No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11030.122342136495225No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10940.12134387790188228No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10780.11956919595816189No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT10690.11857093736481915No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10620.11779451401444146No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCGTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA10270.1139123972625531No Hit
CAGTACCCTCAGGACGTTCGGCCAAGGGACCAAGGTAGAACTCAAACGAACTGTGGCTGCACCATCTGTCTTCAT10150.1125813858047628No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA10140.11247046818328027No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9900.10980844526769969No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTTACT9870.1094756924032521No Hit
GTAATATACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATATGCAGATACA9790.10858835143139191No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9780.10847743380990937No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9770.10836651618842685No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG9750.10814468094546181No Hit
GTCTCCATCGTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAA9700.1075900928380492No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9610.10659183424470645No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA9540.10581541089432878No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTTCTACTGTCAACAGACTCACAGTACCCTCAGGACGTT9510.1054826580298812No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9340.10359705846467827No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9270.1028206351143006No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCAGAGATCAGGAGAGTAGGGGCTTTCCCCGGTTTCTG9260.1027097174928181No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9210.10215512938540547No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9190.10193329414244041No Hit
CCATCGTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9140.10137870603502779No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9080.10071320030613264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)