FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005578430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901570
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31040.3442882970817574No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22320.24756813114899562No Hit
CTCCTGATCTCTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT20630.22882305311844892No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20310.22527368923100813No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20160.22360992490877024No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19000.21074348081679736No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18210.20198098871967793No Hit
GATCAGGAGAGTAGGGGCTTTCCCCGGTTTCTGCTGATACCAGTTTACAT17300.19188748516476814No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17290.1917765675432856No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAACTATGTAAACTGGTATCAGCAGAAA17140.19011280322104773No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16940.18789445079139724No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16460.18257040496023602No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16350.18135031112392824No Hit
GAGTAAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15970.1771354415075923No Hit
GGGTACTGTGAGTCTGTTGACAGTAGAAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTAAAAT15660.17369699524163404No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15220.16881661989640293No Hit
GTGCAGCCACAGTTCGTTTGAGTTCTACCTTGGTCCCTTGGCCGAACGTC15200.16859478465343788No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14650.16249431547189902No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCTACCTTGGTCCCTTGGCCGAACGTCCTGAGGGTA14540.16127422163559124No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14410.15983229255631842No Hit
GATTTTACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTTCTACTGTCAACAGACTCACAGTACC14350.15916678682742327No Hit
GGGTACAACCAGCTGCTATGTGATGGTCCGGCCTTGCGCCTTTGATTTCTGGGGCCAAGGGACAATGGTCACCGT14000.1552846700755349No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13970.1549519172110873No Hit
AGGTAGAACTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13780.15284448240291934No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13710.15206805905254167No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13640.151291635702164No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13610.1509588828377164No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13460.14929511851547855No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13360.14818594230065332No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13280.1472986013287931No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13210.14652217797841544No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12500.13864702685315616No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12390.13742693301684838No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12360.1370941801524008No Hit
GTTCTACCTTGGTCCCTTGGCCGAACGTCCTGAGGGTACTGTGAGTCTGTTGACAGTAGAAAGTTGCAGAATCTT12220.13554133345164546No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12140.13465399247978527No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12060.13376665150792508No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11780.13066095810641437No Hit
ACCTTGGTCCCTTGGCCGAACGTCCTGAGGGTACTGTGAGTCTGTTGACA11780.13066095810641437No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11590.1285535232982464No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11420.12666792373304347No Hit
GAGTAGGGGCTTTCCCCGGTTTCTGCTGATACCAGTTTACATAGTTGCTA11390.1263351708685959No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11230.12456048892487549No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTTACT11130.12345131271005025No Hit
ATGTAAACTGGTATCAGCAGAAACCGGGGAAAGCCCCTACTCTCCTGATC11080.12289672460263762No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11070.1227858069811551No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCGTCC11040.12245305411670752No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10920.12112204265891723No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC10830.1201237840655745No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10790.11968011357964439No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10660.11823818450037157No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG10520.11668533779961622No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGG10390.1152434087203434No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA10250.11369056201958805No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9990.1108067038610424No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9760.10825559856694432No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCGTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA9570.10614816375877636No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9570.10614816375877636No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTTCATTCAGTGAT9530.10570449327284627No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9370.10392981132912586No Hit
GTCTCCATCGTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAA9320.10337522322171325No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9230.10237696462837051No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9140.10137870603502779No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9130.10126778841354526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)