FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415485
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14630.3521186083733468No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10920.26282537275713924No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10570.25440148260466683No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTG10420.25079124396789293No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAACGTTCAGCG10350.24910646593739846No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9830.2365909719965823No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9370.22551957351047572No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA9320.22431616063155108No Hit
GGATATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATC9270.22311274775262646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8720.20987520608445553No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTGAATGGGAAA8710.2096345235086706No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC8530.20530223714454193No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8270.19904449017413384No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8240.19832244244677907No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8140.19591561668892982No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8140.19591561668892982No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT8020.19302742577951068No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7910.1903799174458765No Hit
GTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTG7490.1802712492629096No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7460.17954920153555484No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6970.16775575532209347No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6750.16246073865482508No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6740.16222005607904016No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6710.16149800835168537No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6660.16029459547276076No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6640.1598132303211909No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6480.15596230910863207No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAACGTTCAGCGGCAGTGCA6430.15475889622970745No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6380.1535554833507828No Hit
GACTACCAGTTTCCCATTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT6250.15042660986557876No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC6250.15042660986557876No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6170.1485011492592994No Hit
CTGGTAGTCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAATGAAATCTGTC6150.1480197841077295No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6020.1448909106225255No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5990.14416886289517072No Hit
GATTTCATTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGACTACCAGTTTC5890.14176203713732144No Hit
GTGGGGTCCCATCAACGTTCAGCGGCAGTGCATCTGGGACAGATTTCATT5790.13935521137947218No Hit
GAATGAAATCTGTCCCAGATGCACTGCCGCTGAACGTTGATGGGACCCCA5690.13694838562162293No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5510.13261609925749424No Hit
GACCTGCACCGTCTCGGGGTTCTCACTCAGCGATACTAAACTGGGTGTGA5430.13069063865121486No Hit
GCTAATATCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG5420.13044995607542992No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAATGAAATCTGTCCCAGATGCA5320.12804313031758066No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5180.12467357425659169No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5170.12443289168080676No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5150.12395152652923691No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5100.1227481136503123No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5020.12082265304403288No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5000.12034128789246301No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGCAG4960.11937855758932332No Hit
GTCCCAGATGCACTGCCGCTGAACGTTGATGGGACCCCACTTTGCAAAGT4940.11889719243775346No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4860.11697173183147407No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT4810.11576831895254944No Hit
ATTGTCAACAGACTACCAGTTTCCCATTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTG4740.11408354092205496No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC4720.11360217577048509No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4580.11023261970949613No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4300.10349350758751821No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4290.10325282501173327No Hit
GGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC4260.10253077728437848No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4250.10229009470859357No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC4230.1018087295570237No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4230.1018087295570237No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT4200.10108668182966893No Hit
CTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCA4180.10060531667809909No Hit
CTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGATGACCCAGTC4170.10036463410231415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7950.044.483921
TATCAAC8700.040.2205242
TCAACGC9050.039.4089434
CAACGCA9150.038.9782375
GTGGTAT801.713488E-938.6265141
AACGCAG9550.037.3456466
AGAGTAC9950.036.19332511
CGCAGAG9900.036.0253458
CAGAGTA10250.035.1297810
GCAGAGT10550.034.130839
ACGCAGA10600.033.9698337
TGTTAGA4850.033.1771770
AGTACGG10450.031.18328513
ACGGGAT450.00474868930.49399416
TACGGGT450.004751486730.49032215
GAGTACG10850.030.34981512
ACGGGGC1302.0918378E-1029.02793516
ACGGGAA605.598251E-428.58811816
TACGGGA4350.028.38754315
GTGTAGG854.587495E-628.2756181
Produced by FastQC (version 0.11.5)