FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841969
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAAGGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATTGTGAGAGTGAATTCTGTC33500.3978768814528801No Hit
CCTTAATAGTTACCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT32260.38314949837820633No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24070.28587750855435295No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19500.23159997577107946No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17820.21164674708926337No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16550.1965630563595572No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16460.1954941333944599No Hit
GTCTCCACCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCA15470.1837359807783897No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA15390.18278582703163657No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15270.18136059641150684No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14920.1772036737694618No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14580.17316552034576094No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCACTTATTTAGCCTGGTATCAGCAGAAA14440.17150275128894293No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT14360.1705525975421898No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC14270.16948367457709249No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12720.1510744457337503No Hit
GGGTAACTATTAAGGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTG12490.148342753711835No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12450.14786767683845842No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12330.1464424462183287No Hit
TAGTTACCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT12210.145017215598199No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11730.13931629311768012No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11720.13919752389933596No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11340.13468429360225853No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11150.13242767845371978No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11130.1321901400170315No Hit
GGCCAAAAGTGTACATGGGGTAACTATTAAGGTGTTGACAGTAATAAGTT11120.13207137079868736No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCACCCTTC10890.12933967877677208No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10760.12779567893829819No Hit
TATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGAT10570.1255390637897595No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10180.1209070642743379No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10140.12043198740096131No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGA10140.12043198740096131No Hit
GTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAATAAGTGCTAATG9900.11758152616070187No Hit
GTAATACACGGCCGTGTCAGCGGCGGTCACAGACCTCAGTTTCAGGGAGA9800.11639383397726044No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9630.11437475726541No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9570.11366214195534514No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9550.11342460351865687No Hit
GAATTCACTCTCACAATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACACCTTAATAGTTAC8830.10487321979787854No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG8830.10487321979787854No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8780.10427937370615782No Hit
GTGTACATGGGGTAACTATTAAGGTGTTGACAGTAATAAGTTGCAAAATC8570.10178522012093082No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8520.1011913740292101No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8500.1009538355925218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19350.040.4815141
TATCAAC22350.034.894042
TCGACGT1201.8189894E-1234.39688529
ATCAACG23000.034.0572743
GTGGTAT1950.033.4750981
TCAACGC23400.033.4750984
CAACGCA23800.033.20125
AACGCAG24300.032.5122536
CGACGTC1208.0035534E-1131.53610430
CTCGACG1208.0035534E-1131.52672828
TCGTATG1001.4149919E-831.12563340
AGAGTAC26000.030.25613411
ACGCAGA26400.029.9278287
CGCAGAG26450.029.8712548
CAGAGTA26350.029.85424810
ACGTAGA605.5398373E-428.64363126
ACGAGAC1452.3646862E-1128.4342622
GCAGAGT28000.028.0949829
GACGTCA1353.1468517E-1028.04543731
AGTACGG22700.027.54208613
Produced by FastQC (version 0.11.5)