FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005578435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198489
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGTGGCCGCTCACATTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT25820.2154379389381129No Hit
CTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT24790.2068437841315189No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22700.1894051593297894No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20470.1707983969815326No Hit
GTAATACACACCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGCAGATACA20230.1687958754732No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19150.15978452868570342No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGAATACCAGTGGCCCTGGT15360.12816137653328483No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15290.12757730776002116No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACATTCTCCT13940.11631312427565044No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACATTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTAACTTAGCCTGGTA13260.1106393133353748No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13030.10872023022322273No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12930.1078858462614175No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT12500.10429799522565497No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12000.10012607541662877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)