FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472829
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT17450.36905519754498983No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8810.1863252888464963No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA8480.1793460215003733No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8400.17765407790131316No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC5980.12647278402974435No Hit
GAGTATTAGCAGCTACTTAACCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC5970.12626129107986186No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAACCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT5880.1243578545309192No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG5690.1203394884831514No Hit
GTACCAGACCCACTGCCAATGAACCTGCCTGGGACGCCAGTGGCCCTGTT5500.11632112243538362No Hit
GTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAACCTGGTA5490.1161096294855011No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5260.11124529163820324No Hit
GCATAGCACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAACTGTGGCTGCACCATCTGTCTTCAT5210.11018782688879067No Hit
GTCTGGTACAGACTTCACTCTCACCATCAACAGCCTAGAGCCTGAAGATT5090.10764991149020046No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5050.10680393969067041No Hit
GTGCTATGCTGCTGACAGGAATAAACTGCAAAATCTTCAGGCTCTAGGCTGTTGATGGTGAGAGTGAAGTCTGTA4960.10490050314172776No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4840.10236258774313758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATG100.0076067387141.6088370
TATACAC3400.049.8514023
AAGGGGG4250.049.3840269
CTTATAC3450.049.139311
TTATACA3700.045.814242
CTCGTAT3800.045.04417839
TCGTATG3950.044.3743540
GTGGTAT1550.042.8921
TATCTCG3900.042.63978636
GCCTATC3800.042.57907533
CGTATGC4200.041.8658141
TGGTATC1600.041.5472262
TATGCCG4200.041.31235543
GCCGTCT4300.040.8341246
AAAGGGG4650.040.6182168
ATCTCCG4200.040.355910
AGGGGGG8250.040.3370670
TACACAT4300.040.1902775
CCTATCT4150.039.88160334
ACGAGAC4350.039.7661722
Produced by FastQC (version 0.11.5)