FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472829
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33460.7076554103068974No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8880.18780573949567392No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA8050.1702518246554251No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6140.12985667122786462No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5910.12499233338056676No Hit
GAGTATTAGCAGCTACTTAACCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC5660.11970500963350388No Hit
GTCTGGTACAGACTTCACTCTCACCATCAACAGCCTAGAGCCTGAAGATT5500.11632112243538362No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5490.1161096294855011No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC5430.114840671786206No Hit
GTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAACCTGGTA5400.11420619293655847No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG5290.11187977048785079No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAACCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT5290.11187977048785079No Hit
GTACCAGACCCACTGCCAATGAACCTGCCTGGGACGCCAGTGGCCCTGTT4810.10172810889349003No Hit
GTGCTATGCTGCTGACAGGAATAAACTGCAAAATCTTCAGGCTCTAGGCTGTTGATGGTGAGAGTGAAGTCTGTA4780.10109363004384249No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC4770.10088213709395998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG150.002733650266.451083
GTGGTAT2050.042.197731
GTATCAA11900.036.3467861
TGGTATC2300.036.1338272
GGTATCA5150.033.5943071
TATCAAC12900.032.4700242
TCAACGC13450.031.6165034
ATCAACG13500.031.5027373
CAACGCA13550.031.3864885
AACGCAG13550.031.3864886
AGAGTAC14400.029.76769611
CGCAGAG15200.027.9794038
GCAGAGT15350.027.9224419
ATTAGAG606.7515264E-427.687953
AGTAAGT500.007624084627.67819642
AGTACGG14700.026.89971213
GAGTACG14900.026.31562812
ACGCAGA16600.025.6196967
ACGGGGA4200.024.53659216
TACGGGG8400.024.531415
Produced by FastQC (version 0.11.5)