EGAF00005578441 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005578441
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	534745
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTACTCATTTGCAGAAACA	1242	0.23226023618734162	No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT	1105	0.20664054829872183	No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT	1087	0.20327445791919513	No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC	1049	0.19616826711797214	No Hit
GTTAGTAGCCGCTTGGCCTGGTATCAGCAGAGACCAGGCAAAGCCCCTAA	1000	0.18700502108481612	No Hit
GTATAATAATTTCCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT	996	0.18625700100047685	No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC	974	0.1821428905366109	No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT	923	0.1726056344612853	No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT	911	0.1703615742082675	No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG	884	0.16531243863897746	No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT	862	0.1611983281751115	No Hit
TTATTATACTGTTGGCAGTAATAAGTTGCAAGATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC	851	0.15914127294317854	No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC	833	0.15577518256365183	No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG	794	0.148481986741344	No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC	784	0.14661193653049584	No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG	759	0.14193681100337543	No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA	736	0.13763569551842467	No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG	703	0.13146452982262574	No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG	698	0.13052950471720168	No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT	686	0.12828544446418386	No Hit
CCTTCCACCCTGTCTGCTTCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACACTGTTAGTAGCCG	677	0.12660239927442052	No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC	671	0.12548036914791164	No Hit
GTTTATTACTGTGCGACATCCAGAGAGAATTACGATATTTGGAGACATCC	651	0.12174026872621531	No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC	643	0.12024422855753678	No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG	640	0.11968321349428233	No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG	630	0.11781316328343416	No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT	624	0.11669113315692525	No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG	613	0.11463407792499229	No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT	607	0.1135120477984834	No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTGCAACTTATTACTGCCAACAGTATAATAATTTC	600	0.11220301265088967	No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTG	598	0.11182900260872004	No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC	598	0.11182900260872004	No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT	597	0.11164199758763524	No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTAGCAACTATGCCATGACATGGGTCCGCCAGGCTCCAGGGAAGGGG	592	0.11070697248221115	No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG	586	0.10958494235570225	No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT	585	0.10939793733461745	No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT	573	0.10715387708159965	No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT	568	0.10621885197617557	No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT	546	0.1021047415123096	No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA	544	0.10173073147013997	No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT	543	0.10154372644905515	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGAGCG	20	0.0075201094	51.463577	3
GTATCAA	1075	0.0	42.132263	1
TGTGTAT	90	3.6379788E-12	41.933285	12
ACGGGCT	80	1.7262209E-9	38.6049	16
CTCGTAT	100	9.094947E-12	38.024414	39
GCCGTCT	105	1.4551915E-11	36.426502	46
TACGGGC	170	0.0	36.330627	15
TCGTATG	105	1.6370905E-11	36.247875	40
TATCAAC	1245	0.0	36.100285	2
GTGTCCA	555	0.0	35.96625	70
CGTATGC	110	2.7284841E-11	34.632904	41
ATCAACG	1335	0.0	33.66656	3
TCAACGC	1335	0.0	33.66656	4
TGCCGTC	115	4.3655746E-11	33.24008	45
GCTAATG	65	1.8084083E-5	33.02563	66
TATACAC	125	3.6379788E-12	32.936687	3
CTTATAC	115	5.0931703E-11	32.82042	1
GCTAATC	105	6.530172E-10	32.801044	33
CAACGCA	1380	0.0	32.568737	5
AACGCAG	1385	0.0	32.45116	6

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content