FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005579074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005579074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96429904
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAATTCAGACCAATA2584390.2680071111550624No Hit
TAGCCCTCAGCGTGAA2120790.21993073849788342No Hit
ATCCAGAGTACGGTTT1509940.15658420649262494No Hit
TATCGAGAGTAGTCGG1493260.1548544526187644No Hit
CCGTAGGGTAACTGAA1481550.15364009902986112No Hit
TTTGGTTCAGCGTGAA1389250.14406837945208367No Hit
CCACGTTAGTGTCGGA1362520.14129641775854096No Hit
TTGTCTAAGGGTTCCC1319310.13681544264526074No Hit
GGCGTTGGTTGCAGAG1263220.13099878228645753No Hit
TCACCTGCATCACAAC1240230.12861466708501545No Hit
GCGCATGTCTTTCGAT1226110.12715039102392967No Hit
ATTCGTTTCGGTTGTA1189340.12333725853341097No Hit
CTCTCGAAGAGACTCG1088130.11284155172445261No Hit
TCGCCTAGTATCTAGC1061330.11006233087196686No Hit
TTACGTTCAGAACGAC1048810.1087639784438653No Hit
GGTACCGGTAGGGTCA1047410.1086187952650041No Hit
GGAGTAGTCTATCTCA1037560.10759732789944497No Hit
GAGACTTAGGTCCTCG1018450.10561557750798964No Hit
TAGACTGAGGAGGCGA1009750.10471336775363792No Hit
GCCAGCATCCCAGTAA983660.1020077755132889No Hit
ATATTCCCACGTTACA982250.10186155531172154No Hit
TCGAGCGGTTCTTTGT968050.10038898306898657No Hit
ACCAAACAGGTACATA967060.1002863178210776No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGCG21100.010.0008512
GCCTGGC34900.010.000852
CTCCGCC3450.09.9997877
TTCGCTG1200.09.9997877
CCGGAGC13950.09.9997364
ATCTAGG15850.09.9997354
CGGCATG4150.09.9996325
CTGTGCC2500.09.9996326
GTTGGGC208850.09.9960621
TCCTGCT84600.09.994942
GATGGCC234800.09.9944621
TTACCGC379050.09.9942551
ATCCTGC176200.09.9923371
TGCTTTA253800.09.9909991
TACCCTG230950.09.9900251
TCGCTGA46900.09.9891738
TATTGCT290150.09.9887881
CGCTGGA204600.09.988631
AGCCTCT275850.09.9881611
GAAATGA257600.09.9872631
Produced by FastQC (version 0.11.5)