FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005686633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005686633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19751343
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC41750.020.2572571
CGCGATC20200.018.3293863
GCGCGAT18850.017.6382882
ACCGCAC38150.013.3262021
ACCGTGC50800.012.53309251
GGCGCGA30450.011.5467991
ACTGCGC45750.011.5278691
ACTTTAC115300.011.4559911
GCGATCT40350.011.4112424
CCGCGCC82700.010.7400382
ACTTTAT168550.010.3455711
CGCGCCC65650.010.1227383
ACTTCAC153550.09.5614961
GCTTTAC92100.09.5439741
CGCCCGG70800.09.52055
ACTTAAC87050.09.4426631
ACTGCAC191900.09.1115021
GCTTCAC142300.08.9484341
ACCTCAC144450.08.8810311
CTCACGC94350.08.8044213
Produced by FastQC (version 0.11.5)