FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005686651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005686651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35208996
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC42950.015.42719346-47
TATGCCG43450.015.14040248-49
TCGTATG45500.015.08433344-45
CTCGTAT47650.014.10467944-45
TCTCGTA60000.011.35974242-43
AGATCGG251300.010.58558594-95
TAGATCG93300.010.1828194-95
AATCTCG64700.010.168140-41
GATCGGA226750.010.1185794-95
ATCTCGT69000.09.77478242-43
ATGCCGT76100.09.42474648-49
ATCGGAA217550.09.17084994-95
GTCACGT92650.08.2031928-29
AGTCACG89650.08.00082728-29
TCACGTC98800.07.523938730-31
GCCGTCT100950.07.292968350-51
TCGGAAG227750.07.289674394-95
TGCCGTC102150.07.253793250-51
GTATGCC104050.07.098460746-47
CGTCTGA100100.07.09365416-17
Produced by FastQC (version 0.11.5)