FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005686652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005686652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35208996
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA34200.019.30325746-47
GCCGTAT43750.015.46958646-47
CGTATCA47400.014.57856748-49
CCGTATC50800.013.50934648-49
TCGCCGT53750.012.724256544-45
AGATCGG260250.010.3317194-95
TGGTCGC65800.010.32195442-43
GTCGCCG67500.010.27300444-45
GAGCGTC132500.010.144559
GATCGGA231000.010.04613694-95
TAGATCG86350.09.38012294-95
AGAGCGT146000.09.23905858
ATCGGAA218900.09.10400894-95
GGTCGCC78300.08.91676642-43
GTGGTCG85750.08.47427140-41
TCGTGTA81900.08.322714-15
AAGAGCG171800.08.1280517
GCGTCGT84800.08.06548410-11
CGTCGTG86050.07.92076212-13
CGTGTAG88050.07.90322714-15
Produced by FastQC (version 0.11.5)