FastQCFastQC Report
Tue 4 Apr 2023
EGAF00005687010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93478348
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT2665130.2851066644866253TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA1434710.15348046159309534No Hit
GGGGGCGATAGGGTGGCCTGGGCTTGGGCAGGCTGGAGGCTGCCTCCCTA1157760.1238532799060591No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT946380.10124055679717404No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)