FastQCFastQC Report
Tue 4 Apr 2023
EGAF00005687016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67824455
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT3399380.5012027004124102TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTAT1273500.18776413315816545TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGCGATAGGGTGGCCTGGGCTTGGGCAGGCTGGAGGCTGCCTCCCTA1138110.16780230670486038No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA922040.13594506583208077No Hit
GCCCAGTGGATAGGATGGAGGAAGGGAAACTTCTAGAAAGCTGCCTGTGT911970.13446035062132086No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA850420.1253854527839553No Hit
CTCTGGTCTGCGGTTTGCGCTGCCCTCCTCCTCTTTCTTCTCCTCGGGGT752400.11093343838885251No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)