FastQCFastQC Report
Tue 4 Apr 2023
EGAF00005687018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107470557
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT4323040.4022534283506133TruSeq Adapter, Index 19 (97% over 38bp)
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA1520740.141502942056958No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTAT1444330.13439308777379835TruSeq Adapter, Index 19 (97% over 38bp)
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1259360.11718186219133488No Hit

[FAIL]Adapter Content

Adapter graph