FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21905901
Sequences flagged as poor quality0
Sequence length32-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC14000.010.8893718
TTCCGAT15900.09.5882577
CCGATCT16950.09.198529
ACGCTCT22750.06.24723531
CTTCCGA28000.05.81621936
CGATCTA7850.00215260925.73788510
CGCTCTT28850.05.28480052
Produced by FastQC (version 0.11.5)