FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13879060
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN290710.20945943024959904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8750.022.7803548
CTCGTAT10450.019.24120944
TCGTATG11000.018.63243945
TATCTCG11000.018.1774541
CGTATGC11350.017.7906546
TCTCGTA11750.017.08020643
ATGCCGT12800.016.70451249
TGCCGTC13650.016.21911450
GCCGTCT13800.015.82803451
CGCTCAT12600.015.71339134
ACCGCTC13500.014.63914332
ATCTCGT14200.014.35047942
GATCGGA16250.012.9770171
CCGCTCA17600.011.8198733
ATCGGAA18450.011.7963592
CCGTCTT19100.011.09744652
ACACGTC19000.011.09604113
CACCGCT19400.010.88222531
GTCACCG18800.010.85406229
CGTCTGA20700.010.349153516
Produced by FastQC (version 0.11.5)