FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13879060
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN304480.21938085144094774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAT6750.00644754767.82646470
GCGTCGG9450.00236183066.83262870
AGCGCGT6950.0084390456.047810668
GCTCGGC14850.00131641085.533864570
AGCGTCG13752.512228E-65.489057558
CGTCGTG12508.741299E-55.209141360
GCGTCGT11852.308185E-45.1727659
Produced by FastQC (version 0.11.5)