FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48373039
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATGA491450.0125.090399
AACGTGA288850.0123.882579
ACGATGA305750.0123.674059
ATCGTGA208950.0123.132049
GCGATGA120500.0122.489319
ACGTTGA218950.0122.40869
CCAATGA355450.0121.678039
TCGATGA186000.0120.86389
CGAATGA256450.0120.7918559
CCGATGA167150.0119.747789
AGACTGA324400.0118.19539
CAACTGA296700.0117.941659
ACGCTGA169350.0117.550049
CCTATGA204150.0116.7588659
AGATTGA362600.0116.519669
TCGTTGA130950.0116.036139
GCGTTGA87050.0112.9271559
GCGCTGA65900.0112.867639
AGCCTGA154450.0112.837659
CCGTTGA76000.0112.3670969
Produced by FastQC (version 0.11.5)