FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005687521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66725237
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGA311400.0129.825249
ACGATGA461200.0128.538999
ATCGTGA268050.0128.347969
CCAATGA532100.0127.9640969
AACGTGA390650.0126.961589
GCGATGA162350.0126.8516169
CGAATGA380000.0126.303999
ATCATGA646550.0126.199919
TCGTTGA168100.0126.134899
CCGATGA225400.0126.101439
TCGATGA251900.0125.8140269
AGACTGA439550.0125.783179
GGATTGA216800.0125.686939
ACGCTGA224600.0124.7109159
AGATTGA510900.0124.704089
CCTATGA297800.0123.8266459
AGCCTGA204950.0123.404269
CGTATGA165350.0123.23499
GACGTGA139650.0122.9705669
CACGTGA151850.0122.065539
Produced by FastQC (version 0.11.5)