FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22158538
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG1800.001344017513.49590841
CCGTATC15450.010.55150638
AGTTCCG16900.010.46240234
GATCGGA17300.09.8200261
TTCCGTA16850.09.67276936
TATGCCG12850.09.46961248
TCCGTAT17650.09.43177737
TATCTCG13100.09.27199741
GTTCCGT17100.09.12448835
ATCGGAA20950.08.2736412
CGTATCG5101.01180114E-48.16243739
CGTCTGA25750.07.13393216
CGTATCT19300.06.830226439
CTCGTAT18750.06.667476744
CGTCGAA5200.0090289486.6674431
TCGTATG16855.456968E-126.39079245
ATCTCGT21400.06.1636642
TCTCGTA18055.456968E-126.154985443
ACACGTC30350.05.93847513
CGTATGC17701.200533E-105.8889446
Produced by FastQC (version 0.11.5)