FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18261934
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN808750.44286109017807207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG5101.2720193E-810.57003339
CATCGCG4251.5868692E-48.79457541
GCGCATC10700.08.78283838
TAATGCG12300.08.62656934
AATGCGC12500.08.243752535
ATGCGCA13200.07.57600436
TGCGCAT12950.07.48977937
TATGCCG7056.1185165E-57.01906148
TCGCTCG6750.0063233486.218650358
ATCGATT13850.0045612095.84319970
TCGACCT13900.0047475425.8221870
GCCGGTA8750.00250939125.2576145
CCCGCGG16700.0061509375.249840370
Produced by FastQC (version 0.11.5)