FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17014142
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN996470.5856716136493982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC12850.017.25608438
AATGCGC13850.015.83085735
TAATGCG13750.015.65562434
TATGCCG9100.015.60985948
ATGCGCA14850.014.81699636
TGCGCAT15600.014.80186537
CTCGTAT8950.013.16268944
CGTATGC11400.011.07263346
TCTCGTA11350.010.62626143
CGCATCG6606.548362E-1110.22666139
TCGTATG11250.09.91375645
CGCATCT15650.09.70389239
ATGCCGT14250.08.28068849
ATCTCGT16100.07.874200342
GCATCGC9202.3907342E-77.003302640
CGTCTGA32850.06.78107916
TGCCGTC16501.8189894E-126.76718550
CATCGCG5750.00386348886.519687741
GCCGTCT15554.292815E-106.301207551
GTATGCC28950.05.97177947
Produced by FastQC (version 0.11.5)