FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5622270
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG2900.002019361812.78506870
TTACTCG4050.00203121110.4625670
AGTTACG2350.00997136310.32183634
GCCGGTA9603.4317964E-76.859039335
GTACGCA8954.0742903E-56.1981726
ACTGCGA11451.0291824E-66.075171549
TCGAACC6300.0084248746.04865912
TGCGAAT9509.1428636E-55.86245851
CCGGTAC10901.9563013E-55.723347736
CGGTACA10305.0572562E-55.720973537
AGTACGC9951.8831773E-45.575188625
GCGAATG10252.697495E-45.436657452
AACTGCG11051.397881E-45.34881748
TACGCAC10504.1068468E-45.27817227
AAGTACG12008.966024E-55.20055924
AGTCACG9500.00361033065.106592734
CGTCGGC11058.3227735E-45.020192625
Produced by FastQC (version 0.11.5)