FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11196747
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCCG5957.1390816E-57.590597645
TAAGCGT7000.00372585545.950398441
GTACGCA21300.05.864884426
GCCGGTA23300.05.656309635
ACGAACG8050.00286853875.60083835
GCGACAC15051.4343095E-75.528626412
TACCGCT8200.00354387365.50170340
AGTACGC22107.2759576E-125.49556525
TACGCAC23153.274181E-115.2413627
TTGCGCG9300.00269712375.227364543
CGGTACA24251.2551027E-105.006851737
Produced by FastQC (version 0.11.5)