FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14660311
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG13550.020.9838245
TATCTCG14200.018.75525541
TATGCCG15100.018.64666748
CTCGTAT14600.018.51189244
TCTCGTA17500.016.22514743
CGTATGC18400.015.65442146
ATCGGAA21000.013.7935952
GATCGGA22300.012.6815531
ATCTCGT22300.012.41448542
CGCTCAT24250.011.668529534
GTATGCC26550.010.85710547
ATGCCGT28650.010.44437649
CCGTCTT29400.010.33075152
CGTCTGA29750.010.084702516
ACCGCTC29150.09.34905232
GCCGTCT34050.08.49973151
CACGTCT34800.07.927624714
TGCCGTC37650.07.770908450
TCGGAAG41600.07.377663
CACCGCT40300.07.18934931
Produced by FastQC (version 0.11.5)