FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12884675
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT5650.0025983618.6566670
TATCTCG7854.716636E-56.599467841
CTCGTAT10803.9294173E-76.40997544
GTACGCA26000.06.223903726
AGTACGC26200.05.782132625
TACGCAC26950.05.743483527
TATGCCG9901.6951717E-45.616455648
CGGTACA28850.05.61456337
TCGTATG9901.7700499E-45.59954245
TCTCGTA12951.0030975E-55.341033543
TGCGAAT28850.05.322297651
AAGTACG31700.05.321962424
GCCGGTA30200.05.24572735
CGGCATA8650.00736710855.169544718
GTCCGGT15501.7371676E-65.104995330
ACTGCGA32350.05.05575349
AACTGCG36050.05.01274548
Produced by FastQC (version 0.11.5)