FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795096
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA252.4634833E-455.6643752
CGCGATA306.140261E-446.25882339
CGACCGT404.621973E-543.70796662
CGACGTA404.83065E-543.3821741
CGGATCG400.002535943434.66702715
ATTCGCG703.6569625E-529.79768650
TTCGCGT755.8390044E-527.8212951
CTATCGG658.158339E-426.8104956
TAGGGGG8700.026.77225150
TACGCGT658.414092E-426.66917231
ATAGGGG4550.025.97456649
GCGTTCG750.001754719623.50995867
TTATCGG750.001754719623.50995867
CTACGCA750.001870102223.25401358
TAGTGCG750.001898665723.19349152
TATAGGG5100.023.1669148
CCGATAA902.0511704E-423.123637
TATAACG952.8927476E-421.97831753
CTAGCGC800.002791284621.7050231
GACCGTT1004.035464E-420.92156657
Produced by FastQC (version 0.11.5)