FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217066
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT800.002806422521.6850836
TAAACGA1506.958544E-720.81485614
CGCGCTT1408.86144E-619.8371073
TAGCGTC1258.705817E-418.66470569
CTTGACG2000.002377175516.06492670
GGTATCG1300.0024356516.00709329
ACGTTAT1350.003130681315.4156318
TCGTAAA1656.9765135E-414.71757612
CGCGACT1656.9896533E-414.71392321
CGACTAG1951.5291359E-414.22884823
CGCTTCG1750.001079515813.89256444
CAATCCG1500.00627940213.8822124
TATCCGC1500.006284113.8806445
GTACGAA1500.00629068213.8784536
TTGCGTT1800.001282295213.57938161
TGTACGA2304.053966E-513.57613335
CCGTATG1550.0078175313.43015235
CCATACG1850.001655838713.12648712
CGCCTAA1600.00964300213.01016734
TCGTTGG2902.6105463E-511.98447248
Produced by FastQC (version 0.11.5)