FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18618499
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAAT43150.05.80661951
GTACGCA41950.05.794054526
AGTACGC42950.05.578277625
GCGAATG46700.05.441390552
CTGCGAA52050.05.34715450
CGGTACA46900.05.1799837
TACGCAC47550.05.1797527
GCCGGTA47650.05.17036635
Produced by FastQC (version 0.11.5)