FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25693312
Sequences flagged as poor quality0
Sequence length32-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC11700.012.6650888
TATGCCG11750.010.96395748
CCGTATC15550.010.67615938
CCGATCT13950.010.3753629
TATCTCG14150.09.54828141
AGTTCCG17050.09.51746234
TTCCGAT17100.08.8671297
CTCGTAT14200.08.79916344
CGTATGC16500.08.63746246
TCCGTAT19650.08.4441937
GTTCCGT23300.07.85630835
TCTCGTA15950.07.828547543
TCGTATG16750.07.672473445
TTCCGTA22200.07.62662636
CTTCCGA23000.06.89231166
ATCTCGT20700.06.86603642
CGTATCT22250.06.842455439
TGCCGTC20700.06.73944850
ATGCCGT19450.06.62894849
ACGCTCT21500.06.0932061
Produced by FastQC (version 0.11.5)