FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20636864
Sequences flagged as poor quality0
Sequence length32-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8302.8067006E-98.38432448
CGCGAAG5452.3038284E-47.6033276
CCGCGAA5500.00210969696.9063555
CCCGATC10251.0813201E-66.41890638
CGTCCCG10308.106355E-66.045201335
GTCCCGA11551.3183053E-65.9918636
GATCGCG14502.4631733E-45.85180570
CTCGTAT11608.502138E-65.68672644
CGTATGC12051.5835247E-55.479887546
CCGATCT11101.5811143E-45.30533139
GCGCGCC14250.00751861985.16053970
TCGTATG15501.4367852E-65.15442145
TCTCGTA12201.1469066E-45.11997343
TCCCGAT11703.5058154E-45.029483337
Produced by FastQC (version 0.11.5)