FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9911074
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN228920.23097395902805287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCG8200.025.57528335
TATGCCG8600.024.79450248
TACTCGA8900.023.21974236
TCGTATG10000.022.47473545
CGTATGC10650.020.52249546
TCTCGTA10100.019.39498143
CTCGTAT10350.018.98178144
ACTCGAT12100.017.67030537
ATGCCGT13850.015.711246549
TCGATCT12800.015.68866939
GCCGTCT14750.015.35935751
ATCTCGT13200.014.80273342
CTCGATC14150.014.65613338
TGCCGTC16500.013.4546550
GATCTCG15650.013.10780241
CCGTCTT17450.012.428352
GATCGGA16200.012.0320331
CGATCTC17100.011.7691940
ATCGGAA17600.011.645662
GTATGCC20800.010.88149847
Produced by FastQC (version 0.11.5)