FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15334619
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN192340.12542861351821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG9203.6379788E-128.99117841
TCGTATG11850.08.1927545
TATGCCG11001.5279511E-107.60640248
TCTCGTA12751.8189894E-127.590481843
TGGTCCG11304.955677E-57.132282770
CGTATGC13259.373252E-96.29132346
ATACCGG7307.951163E-46.234753650
CTCGTAT13401.2820237E-86.199649344
GCGTTCG6450.0082777556.05912556
ATCTCGT16105.12955E-106.00230842
ATGCCGT19401.9281288E-105.582712749
TTCGGCG7400.0082838925.53643566
TGCCGTC20401.2187229E-105.491849450
GTACGCA19301.7716957E-95.306963426
TCGAGTG15350.00255197855.250475470
CCGTCTT21504.4929038E-105.23461652
CGAAGAC17705.020612E-45.203860870
AGTACGC19954.118192E-95.134054725
ACCGCTC19401.05483196E-85.114306432
GCCGTCT19252.5789632E-85.103376451
Produced by FastQC (version 0.11.5)