FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14178980
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC2001.120227E-515.55912738
TCGACGG3252.1500455E-914.84591324
CGAATCG2253.1533244E-513.94465250
CGTACGT2851.5104106E-613.39460645
GCGGTAT5300.013.03267835
CGTAGCG3053.4934637E-612.42980414
CGGACTA5256.730261E-1112.0375856
ACGTACG3054.4789558E-511.36881444
AACGCGT3054.766098E-511.29974217
CCTAACG7550.011.00006741
CTACGCG2600.001811576810.60781731
TCGACGC3351.0107459E-410.49409657
ACGACCG4300.002117398410.41069970
TACGCCG3652.5694828E-510.38640225
CGGATCG3351.23595E-410.28764319
CGGTATT8850.010.14957436
GCGATTG9608.376446E-99.90914270
CGCAACG3158.0256275E-49.84688328
GTATGCG5752.1646293E-49.7317470
CGATACG3259.621473E-49.6539950
Produced by FastQC (version 0.11.5)